ABSTRACT
Antiestrogens affect spermatozoa through their action on Leydig and Sertoli cells. Direct effect of antiestrogens namely tamoxifen and centchroman in concentration of 1, 2.5, 5, 10 and 20 micrograms/ml in incubation medium was determined on motility and penetration ability of human spermatozoa. Motility (%) was invariably reduced after 15, 30 and 60 min. of incubation. Addition of 17 beta-estradiol to medium with antagonist caused inhibition of motility in dose related manner. The distance travelled by spermatozoa treated with tamoxifen or centchroman in media was reduced by 30% and addition of estradiol along with antiestrogen reduced it to 50% compared to that of untreated spermatozoa.
Subject(s)
Adult , Centchroman/pharmacology , Estradiol/pharmacology , Estrogen Antagonists/pharmacology , Female , Humans , Male , Sperm Motility/drug effects , Sperm-Ovum Interactions/drug effects , Tamoxifen/pharmacologyABSTRACT
Administration of STS-557 (17 alpha-cyanomethyl-17 beta-hydroxyestra 4,9(10)-dien-3-one; 12 mg/monkey daily) for 4 weeks either alone or in combination with 20 Aet-1 (testosterone-trans-4-n-butyl cyclohexyl carboxylate; code CDB 1781; 40 mg/monkey single administration) had no significant effect on motility and zona free hamster egg penetration by spermatozoa of bonnet monkey, but continuation of the treatment for 12 weeks reduced (in one monkey treated with STS-557) or abolished (one treated with STS-557 and two with STS-557 + 20 Aet-1) the motility as well as zona-free hamster egg penetration (by spermatozoa of all treated monkeys). Motility and the ability to penetrate zona-free hamster egg returned to normalcy after 10 weeks of withdrawal of treatments. Active immunization of monkeys with ovine FSH (4 weeks after booster) had no adverse effect on motility of spermatozoa but none of the zona-free hamster eggs was fertilized. The correlation between motility and the capacity to penetrate the zona-free hamster eggs by monkey spermatozoa varies with the treatment. Such correlation was apparent in monkeys treated with STS-557 but not in monkeys immunized with ovine FSH.
Subject(s)
Animals , Contraceptive Agents, Male/pharmacology , Cricetinae , Female , Fertility/drug effects , Follicle Stimulating Hormone/pharmacology , Macaca radiata , Male , Nandrolone/analogs & derivatives , Ovum/physiology , Sperm Motility/drug effects , Sperm-Ovum Interactions/drug effects , Testosterone/analogs & derivatives , Zona Pellucida/physiologySubject(s)
Humans , Male , Cell Membrane/drug effects , Oligospermia/metabolism , Receptors, Cell Surface/analysis , Sperm-Ovum Interactions/drug effects , Spermatozoa/metabolism , Varicocele/physiopathology , Cell Membrane/metabolism , Methods , Oligosaccharides/metabolism , Orchitis/physiopathology , Horseradish Peroxidase/pharmacokinetics , Receptors, Concanavalin A/analysis , Sperm Agglutination , Sperm Count , Wheat Germ Agglutinins/pharmacokineticsSubject(s)
Humans , Male , Sperm Agglutination , Cell Membrane/immunology , Receptors, Cell Surface/analysis , Sperm-Ovum Interactions/drug effects , Spermatozoa/metabolism , Acrosome/immunology , Acrosome/physiology , Concanavalin A/pharmacokinetics , Lectins/pharmacokinetics , Methods , Oligosaccharides/metabolism , Horseradish Peroxidase/pharmacokinetics , Receptors, Mitogen/analysis , Receptors, Concanavalin A/analysis , Receptors, Concanavalin A/immunology , Sperm Count , Wheat Germ Agglutinins/pharmacokineticsABSTRACT
Se realizaron pruebas in vitro para conocer el efecto del ácido acetilsalicílico (AAS) sobre la movilidad y viabilidad de los espermatozoides, así como su penetración en el moco cervical. Se estudiaron muestras de semen con espermatozoides de movilidad (mayor o igual 40%) y viabilidad (mayor o igual 50%) excelentes; y muestras de moco periovulatorio con score cervical mayor o igual 12. Se utilizaron concentraciones de AAS de 5, 10 y 15 mg/ml y un control de solución Ringer. La movilidad y viabilidad se determinaron en condiciones basales y a las 1,2 y 3 horas de añadirles AAS; la penetración en el moco cervical se midió cada 5 minutos, hasta los 30 minutos. Queda por aclarar el mecanismo por el cual AAS ejerce este efecto si esta acción se mantiene en vivo, y sus posibles efectos desfavorables